NSMCE2 antibody (Middle Region)

Catalog No. ABIN2786320

This Rabbit Polyclonal antibody specifically detects NSMCE2 in WB. It exhibits reactivity toward Human, Mouse, Rabbit, Horse, Guinea Pig and Cow.

Quick Overview for NSMCE2 antibody (Middle Region) (ABIN2786320)

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Reactivity: Human, Mouse, Rabbit, Horse, Guinea Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSMCE2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NSMCE2 Antibody

Binding Specificity: Middle Region

Sequence: KFLALQSKNS DADFQNNEKF VQFKQQLKEL KKQCGLQADR EADGTEGVDE

Predicted Reactivity: Cow: 86%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 79%, Rabbit: 100%

Characteristics: This is a rabbit polyclonal antibody against NSMCE2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NSMCE2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 247 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NSMCE2

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Alternative Name: NSMCE2

Background: NSMCE2 is the E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. NSMCE2 acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
Alias Symbols: C8orf36, FLJ32440, MMS21, NSE2
Protein Interaction Partner: TXLNA, PAN2, UBC, NSMCE1, SMC6, PDS5A, SMC5, WAPAL, PDS5B, STAG2, SMC3, SMC1A, SUMO1, TSNAX, RAD21, ETV1, SUMO2,
Protein Size: 247

Molecular Weight: 28 kDa

Gene ID: 286053

NCBI Accession: NM_173685, NP_775956

UniProt: Q96MF7