FBXO33 antibody (Middle Region)

Catalog No. ABIN2786447

This Rabbit Polyclonal antibody specifically detects FBXO33 in WB. It exhibits reactivity toward Rat, Human, Mouse, Cow, Dog, Guinea Pig, Rabbit, Horse and Zebrafish (Danio rerio).

Quick Overview for FBXO33 antibody (Middle Region) (ABIN2786447)

Target: FBXO33 (F-Box Protein 33 (FBXO33))

Reactivity: Rat, Human, Mouse, Cow, Dog, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO33 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FBXO33 Antibody

Binding Specificity: Middle Region

Sequence: VIDTSGFPDL SDNRNEDPLV LLAWRCTKLS LLAIHGYTVW AHNLIAIARL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against FBXO33. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXO33

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 555 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FBXO33

Target: FBXO33 (F-Box Protein 33 (FBXO33))

Alternative Name: FBXO33

Background: FBXO33 is the substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO33 probably recognizes and binds to phosphorylated target proteins. Recognizes YBX1.Members of the F-box protein family, such as FBXO33, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].
Alias Symbols: Fbx33, c14_5247, BMND12
Protein Interaction Partner: CUL1, YBX1, UBC,
Protein Size: 555

Molecular Weight: 62 kDa

Gene ID: 254170

NCBI Accession: NM_203301, NP_976046

UniProt: Q7Z6M2