RPL5 antibody (N-Term)

Catalog No. ABIN2786525

The Rabbit Polyclonal anti-RPL5 antibody has been validated for WB. It is suitable to detect RPL5 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit and Cow.

Quick Overview for RPL5 antibody (N-Term) (ABIN2786525)

Target: RPL5 (Ribosomal Protein L5 (RPL5))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RPL5 Antibody

Binding Specificity: N-Term

Sequence: RLVIQDKNKY NTPKYRMIVR VTNRDIICQI AYARIEGDMI VCAAYAHELP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RPL5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RPL5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 297 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RPL5

Target: RPL5 (Ribosomal Protein L5 (RPL5))

Alternative Name: RPL5

Background: RPL5 is required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MGC117339, MSTP030, L5, DBA6
Protein Interaction Partner: HUWE1, UBC, PML, HAUS2, CEP250, TUBG1, TP53, SUMO2, SUMO3, STAU1, LGR4, VHL, RPL5, FBXO6, UPF2, TARDBP, IGSF8, ICAM1, CD81, PAN2, UBL4A, NOS2, VCAM1, MDM2, ITGA4, HSP90AB1, HSP90AA1, FN1, CSNK2A1, RPS3, RPL11, NPM1, ESR1, CBX5, EEF2, EEF1G, EEF1A1, DHX9,
Protein Size: 297

Molecular Weight: 34 kDa

Gene ID: 6125

NCBI Accession: NM_000969, NP_000960

UniProt: P46777