RPS15 antibody (Middle Region)

Catalog No. ABIN2786563

The Rabbit Polyclonal anti-RPS15 antibody has been validated for WB. It is suitable to detect RPS15 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit and Zebrafish (Danio rerio). There are 2+ publications available.

Quick Overview for RPS15 antibody (Middle Region) (ABIN2786563)

Target: RPS15 (Ribosomal Protein S15 (RPS15))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS15 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RPS15 Antibody

Binding Specificity: Middle Region

Sequence: GVYNGKTFNQ VEIKPEMIGH YLGEFSITYK PVKHGRPGIG ATHSSRFIPL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RPS15. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPS15

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 145 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RPS15 antibody (ABIN2786563)

Cichocka, Karlik, Plewka, Gawade, Stępień, Świergiel, Gadgil, Raczyńska: "The Novel Role of hnRNP UL1 in Human Cell Nucleoli." in: International journal of biological sciences, Vol. 18, Issue 13, pp. 4809-4823, (2022) (PubMed).

Tiku, Jain, Raz, Nakamura, Heestand, Liu, Späth, Suchiman, Müller, Slagboom, Partridge, Antebi: "Small nucleoli are a cellular hallmark of longevity." in: Nature communications, Vol. 8, pp. 16083, (2018) (PubMed).

Target Details for RPS15

Target: RPS15 (Ribosomal Protein S15 (RPS15))

Alternative Name: RPS15

Background: The specific function of this protein remains unknown.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MGC111130, RIG, S15
Protein Interaction Partner: G2E3, UBC, CEP76, TUBGCP3, TUBG1, TP53, RPS17, RNF2, RPS2, RPS29, RPS25, RPS19, RPS10, RPS9, MDM4, MDM2, ICAM1, CD81, IGSF8, PLA2G12A, SERINC3, RAPGEF1, FN1, SMURF1, ATP6V0A1, ELAVL1, STT3B, MRPL24, PTRH2, ATP6V1D, MRPL2, RPL36, ATP6V0A2, MRPL3, TCIRG1, F
Protein Size: 145

Molecular Weight: 17 kDa

Gene ID: 6209

NCBI Accession: NM_001018, NP_001009

UniProt: P62841

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly