MBP antibody (Middle Region)

Catalog No. ABIN2786586

Product Details anti-MBP Antibody

Target: MBP (Myelin Basic Protein (MBP))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Guinea Pig, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Sequence: FGGDRGAPKR GSGKDSHHPA RTAHYGSLPQ KSHGRTQDEN PVVHFFKNIV

Predicted Reactivity: Cow: 80%, Dog: 87%, Guinea Pig: 79%, Horse: 80%, Human: 100%, Mouse: 93%, Pig: 80%, Rabbit: 93%, Rat: 87%

Characteristics: This is a rabbit polyclonal antibody against MBP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MBP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 304 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin basic protein (MBP Products)

Background: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. MBP induces T-cell proliferation.The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
Alias Symbols: MGC99675
Protein Interaction Partner: CTDSP1, MAP3K3, UBC, PRKCB, MAPK3, MAPK1, ULK1, SQSTM1, PKN1, HIPK2, MNAT1, CDK9, CDK8, CDK7, CCNT1, CCNH, CCNC, MELK, DDX58, MAPK14, AT4G38520, AT4G31860, AT4G28400, ABI1, RPS6KA6, TOPP8, AT5G24940, AT5G10740, AT5G06750, AT3G17250, AT3G02750, AT3G15260,
Protein Size: 304

Molecular Weight: 33 kDa

Gene ID: 4155

NCBI Accession: NM_001025101, NP_001020272

UniProt: P02686