ATP6V1B2 antibody (N-Term)

Catalog No. ABIN2786642

The Rabbit Polyclonal anti-ATP6V1B2 antibody (ABIN2786642) specifically detects ATP6V1B2 in WB. The antibody is reactive with Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Rabbit and Guinea Pig samples.

Quick Overview for ATP6V1B2 antibody (N-Term) (ABIN2786642)

Target: ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1B2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6V1B2 Antibody

Binding Specificity: N-Term

Sequence: VSRNYLSQPR LTYKTVSGVN GPLVILDHVK FPRYAEIVHL TLPDGTKRSG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against ATP6V1B2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATP6V1B2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 511 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATP6V1B2

Target: ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))

Alternative Name: ATP6V1B2

Background: ATP6V1B2 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. ATP6V1B2 is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ATP6B1B2, ATP6B2, HO57, VATB, VPP3, Vma2
Protein Interaction Partner: TCF4, UBC, PBDC1, GMPPB, GANAB, SWAP70, MSH2, JUP, MSH6, XRCC6, CRMP1, HYOU1, SEC24C, CUL2, SUPT5H, SSRP1, PDE3A, CSDE1, PAN2, FN1, SRXN1, YIF1B, UNK, ZFYVE19, TUBA1C, INTS2, TRMT1, ABCF3, ATP6V1H, CCT8, SSSCA1, TUBB4B, UBA2, USP34, ATP6V1F, ZBED1, ZPR1,
Protein Size: 511

Molecular Weight: 56 kDa

Gene ID: 526

NCBI Accession: NM_001693, NP_001684

UniProt: P21281

Pathways: Transition Metal Ion Homeostasis, Proton Transport