PKC iota antibody (Middle Region)

Catalog No. ABIN2786697

This Rabbit Polyclonal antibody specifically detects PKC iota in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Rabbit.

Quick Overview for PKC iota antibody (Middle Region) (ABIN2786697)

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC iota antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKC iota Antibody

Binding Specificity: Middle Region

Sequence: TVIPYNPSSH ESLDQVGEEK EAMNTRESGK ASSSLGLQDF DLLRVIGRGS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PRKCI. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PRKCI

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 596 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PKC iota

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Alternative Name: PRKCI

Background: This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.
Alias Symbols: DXS1179E, MGC26534, PKCI, nPKC-iota
Protein Interaction Partner: PARD6B, PARD6A, UBC, AMOT, SQSTM1, CRX, NPM1, IKBKG, YWHAE, IL1RAP, HSP90AA1, GAB1, CASP8, PARD3, APP, UBD, MAP2K5, MARK2, ARHGAP17, TJP1, ELAVL1, CDK7, MBP, TSC22D1, TTR, FABP4, PARD6G, MARK4, CDC37, RAPGEF2, PNMA1, NIPSNAP1, YWHAZ, YWHAH, MYO10, LLGL1,
Protein Size: 596

Molecular Weight: 68 kDa

Gene ID: 5584

NCBI Accession: NM_002740, NP_002731

UniProt: P41743

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation