PRKAA2 antibody (Middle Region)

Catalog No. ABIN2786849

The Rabbit Polyclonal anti-PRKAA2 antibody has been validated for WB. It is suitable to detect PRKAA2 in samples from Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse, Sheep and Zebrafish (Danio rerio).

Quick Overview for PRKAA2 antibody (Middle Region) (ABIN2786849)

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRKAA2 Antibody

Binding Specificity: Middle Region

Sequence: AYHLIIDNRR IMNQASEFYL ASSPPSGSFM DDSAMHIPPG LKPHPERMPP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PRKAA2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PRKAA2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 552 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRKAA2

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Alternative Name: PRKAA2

Background: The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that
Alias Symbols: AMPK, AMPK2, PRKAA, AMPKa2
Protein Interaction Partner: KCTD1, NUTM1, RFX6, KIF24, RBPMS, APPBP2, ABI2, AIMP2, TRIP6, TCF4, VPS52, PRKAG1, PRKAB2, L3MBTL3, ZNF397, USHBP1, TSC22D4, HMBOX1, CCNB1IP1, HOMEZ, NECAB2, ZBTB8A, NOTCH2NL, KRTAP10-3, NONO, KRT31, KIFC3, GLI1, FOS, AES, PPARG, UBC, HSP90AA1, WWP1, RPTO
Protein Size: 552

Molecular Weight: 62 kDa

Gene ID: 5563

NCBI Accession: NM_006252, NP_006243

UniProt: P54646

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect