CHCHD3 antibody (N-Term)

Catalog No. ABIN2787033

This Rabbit Polyclonal antibody specifically detects CHCHD3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Sheep, Cow, Horse, Rabbit and Guinea Pig and has been mentioned in 2+ publications.

Quick Overview for CHCHD3 antibody (N-Term) (ABIN2787033)

Target: CHCHD3 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 3 (CHCHD3))

Reactivity: Human, Mouse, Rat, Dog, Sheep, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHCHD3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHCHD3 Antibody

Binding Specificity: N-Term

Sequence: RMKESSPSGS KSQRYSGAYG ASVSDEELKR RVAEELALEQ AKKESEDQKR

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against CHCHD3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHCHD3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 227 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHCHD3 antibody (ABIN2787033)

Ott, Ross, Straub, Thiede, Götz, Goosmann, Krischke, Mueller, Krohne, Rudel, Kozjak-Pavlovic: "Sam50 functions in mitochondrial intermembrane space bridging and biogenesis of respiratory complexes." in: Molecular and cellular biology, Vol. 32, Issue 6, pp. 1173-88, (2012) (PubMed).

Darshi, Trinh, Murphy, Taylor: "Targeting and import mechanism of coiled-coil helix coiled-coil helix domain-containing protein 3 (ChChd3) into the mitochondrial intermembrane space." in: The Journal of biological chemistry, Vol. 287, Issue 47, pp. 39480-91, (2012) (PubMed).

Target Details for CHCHD3

Target: CHCHD3 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 3 (CHCHD3))

Alternative Name: CHCHD3

Background: The function of this protein remains unknown.
Alias Symbols: FLJ20420, MINOS3, PPP1R22
Protein Interaction Partner: NOTCH2NL, SPERT, KRT40, SSX2IP, RAB3IP, LZTS2, USHBP1, CCDC33, GMCL1P1, RPGRIP1, FANCL, SAMM50, SPAG5, CALCOCO2, PDE4DIP, BLZF1, TRAF1, TRIM27, GOLGA2, UBC, GPRASP2, MDM2, SUZ12, RNF2, EZH2, PARK2, HDAC8, NPM1, PAN2, PPP1CA, MYC, FMNL1, MDC1, ECT2, SNRPD3
Protein Size: 227

Molecular Weight: 26 kDa

Gene ID: 54927

NCBI Accession: NM_017812, NP_060282

UniProt: Q9NX63