RIC8A antibody (N-Term)

Catalog No. ABIN2787279

The Rabbit Polyclonal anti-RIC8A antibody has been validated for WB. It is suitable to detect RIC8A in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for RIC8A antibody (N-Term) (ABIN2787279)

Target: RIC8A (Resistance To Inhibitors of Cholinesterase 8 Homolog A (C. Elegans) (RIC8A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIC8A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RIC8A Antibody

Binding Specificity: N-Term

Sequence: KLTERVGLYR ERSFPHDVQF FDLRLLFLLT ALRTDVRQQL FQELKGVRLL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RIC8A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RIC8A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 537 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RIC8A

Target: RIC8A (Resistance To Inhibitors of Cholinesterase 8 Homolog A (C. Elegans) (RIC8A))

Alternative Name: RIC8A

Background: RIC8A is a guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins.RIC8A is able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP.RIC8A is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
Alias Symbols: MGC104517, MGC131931, MGC148073, MGC148074, RIC8, synembryn
Protein Interaction Partner: NECAB2, UBQLN1, RABAC1, UBC, EFEMP1, SDCBP, NUTM1, SARNP, WDR61, DPP7, PAPSS2, EZR, UBA1, RPL38, PYGB, PLCG1, MC1R, APEX1, GNAQ, XPO1, GNAI1, UBQLN4, ZNF585B, RIC8A, MAPK8IP3, GNA13, TERF1, GNAI3, GNAI2, GNAS, GNAO1, TUBB2A,
Protein Size: 537

Molecular Weight: 60 kDa

Gene ID: 60626

NCBI Accession: NM_021932, NP_068751

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Asymmetric Protein Localization