POLR1E antibody (N-Term)

Catalog No. ABIN2787316

The Rabbit Polyclonal anti-POLR1E antibody has been validated for WB. It is suitable to detect POLR1E in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for POLR1E antibody (N-Term) (ABIN2787316)

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1E antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR1E Antibody

Binding Specificity: N-Term

Sequence: SPGNMRFTLY ENKDSTNPRK RNQRILAAET DRLSYVGNNF GTGALKCNTL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against POLR1E. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLR1E

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 419 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR1E

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Alternative Name: POLR1E

Background: POLR1E is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.POLR1E is the component of RNA polymerase I which synthesizes ribosomal RNA precursors.POLR1E appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
Alias Symbols: FLJ13390, FLJ13970, PAF53, PRAF1, RP11-405L18.3
Protein Interaction Partner: UBC, RNF2, PAXIP1, CPSF3, ARRB1, CEBPA, SMARCAD1, RPA49, RRN3, POLR1A, SUPT16H, TAF1D, POLR1C, NFKBIB, POLR1B, TAF1C, UBTF, CD3EAP, QPCTL, DDX20, HNRNPUL1, DDX6,
Protein Size: 419

Molecular Weight: 47 kDa

Gene ID: 64425

NCBI Accession: NM_022490, NP_071935