VPS52 antibody (Middle Region)

Catalog No. ABIN2787317

This Rabbit Polyclonal antibody specifically detects VPS52 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for VPS52 antibody (Middle Region) (ABIN2787317)

Target: VPS52 (Vacuolar Protein Sorting 52 Homolog (VPS52))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS52 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VPS52 Antibody

Binding Specificity: Middle Region

Sequence: RYWEQVLALL WPRFELILEM NVQSVRSTDP QRLGGLDTRP HYITRRYAEF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against VPS52. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human VPS52

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 723 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-VPS52 antibody (ABIN2787317)

Harrison, Haga, Pechenick Jowers, Jasim, Cintrat, Gillet, Schmitt-John, Digard, Beard: "Vaccinia virus uses retromer-independent cellular retrograde transport pathways to facilitate the wrapping of intracellular mature virions during viral morphogenesis." in: Journal of virology, (2016) (PubMed).

Target Details for VPS52

Target: VPS52 (Vacuolar Protein Sorting 52 Homolog (VPS52))

Alternative Name: VPS52

Background: This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.
Alias Symbols: ARE1, DKFZp547I194, RP5-1033B10, SAC2, SACM2L, dJ1033B10.5
Protein Interaction Partner: FAM184A, MRPL11, SH2D4A, CCDC146, THAP11, KIAA1217, TBC1D22B, HEATR1, C1orf109, METTL13, ATP6V1D, VPS28, KANK2, TXN2, RNF41, EPM2AIP1, WTAP, USP2, STX11, LMO4, TRAF6, TPM3, TEAD4, RAB4A, PRKAA2, PRKAA1, NOP2, NFKBIB, MFAP1, KIF5B, GOLGA1, DDX6, AAMP, TXLN
Protein Size: 723

Molecular Weight: 82 kDa

Gene ID: 6293

NCBI Accession: NM_022553, NP_072047

UniProt: Q8N1B4