NASP antibody (Middle Region)

Catalog No. ABIN2787368

The Rabbit Polyclonal anti-NASP antibody has been validated for WB and IHC. It is suitable to detect NASP in samples from Human, Rat, Mouse, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Horse and Pig. There is 1 publication available.

Quick Overview for NASP antibody (Middle Region) (ABIN2787368)

Target: NASP (Nuclear Autoantigenic Sperm Protein (Histone-Binding) (NASP))

Reactivity: Human, Rat, Mouse, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NASP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NASP Antibody

Binding Specificity: Middle Region

Sequence: KEAEGSSAEY KKEIEELKEL LPEIREKIED AKESQRSGNV AELALKATLV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against NASP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NASP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 449 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NASP antibody (ABIN2787368)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for NASP

Target: NASP (Nuclear Autoantigenic Sperm Protein (Histone-Binding) (NASP))

Alternative Name: NASP

Background: This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localize
Alias Symbols: DKFZp547F162, FLB7527, FLJ31599, FLJ35510, MGC19722, MGC20372, MGC2297, PRO1999
Protein Interaction Partner: H3F3C, HUWE1, HIST1H3H, H3F3B, UBC, SMAD6, MAT2B, GSPT2, ATG7, TBCD, SNX1, SHMT2, PRKACA, PPP3CA, ATP6V1C1, BAG3, PNKP, ASF1A, STIP1, VCP, CDK2, TERF2IP, TERF2, SUMO2, PTK2, HDAC6, HIST1H3A, Mapk13, HIST1H3E, H3F3A, AIRE, HIST1H4A, LUC7L2, HIST1H3G, HIST2
Protein Size: 449

Molecular Weight: 49 kDa

Gene ID: 4678

NCBI Accession: NM_152298, NP_689511

UniProt: Q5T626