ASAH1 antibody (N-Term)

Catalog No. ABIN2787375

The Rabbit Polyclonal anti-ASAH1 antibody has been validated for WB. It is suitable to detect ASAH1 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Rabbit. There are 2+ publications available.

Quick Overview for ASAH1 antibody (N-Term) (ABIN2787375)

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASAH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ASAH1 Antibody

Binding Specificity: N-Term

Sequence: MPGRSCVALV LLAAAVSCAV AQHAPPWTED CRKSTYPPSG PTYRGAVPWY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ASAH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ASAH1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 395 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ASAH1 antibody (ABIN2787375)

Tan, Liu, Fox, Barth, Sharma, Turner, Awwad, Dewey, Doi, Spitzer, Shah, Morad, Desai, Amin, Zhu, Liao, Yun, Kester, Claxton, Wang, Cabot, Schuchman, Levine, Feith, Loughran: "Acid ceramidase is upregulated in AML and represents a novel therapeutic target." in: Oncotarget, Vol. 7, Issue 50, pp. 83208-83222, (2016) (PubMed).

Elamin, Titz, Dijon, Merg, Geertz, Schneider, Martin, Schlage, Frentzel, Talamo, Phillips, Veljkovic, Ivanov, Vanscheeuwijck, Peitsch, Hoeng: "Quantitative proteomics analysis using 2D-PAGE to investigate the effects of cigarette smoke and aerosol of a prototypic modified risk tobacco product on the lung proteome in C57BL/6 mice." in: Journal of proteomics, Vol. 145, pp. 237-45, (2016) (PubMed).

Target Details for ASAH1

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Alternative Name: ASAH1

Background: This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into
Alias Symbols: AC, ASAH, FLJ21558, FLJ22079, PHP, PHP32, ACDase
Protein Interaction Partner: SRPK1, CAMK1D, FBXO6, UBC, PSMA3, Bub1b, TSC22D1, SETDB1, SMPD1,
Protein Size: 395

Molecular Weight: 44 kDa

Gene ID: 427

NCBI Accession: NM_177924, NP_808592

UniProt: Q13510