PSMD12 antibody (Middle Region)

Catalog No. ABIN2787421

This Rabbit Polyclonal antibody specifically detects PSMD12 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio) and Rabbit.

Quick Overview for PSMD12 antibody (Middle Region) (ABIN2787421)

Target: PSMD12 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 12 (PSMD12))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSMD12 Antibody

Binding Specificity: Middle Region

Sequence: KYKDLLKLFT TMELMRWSTL VEDYGMELRK GSLESPATDV FGSTEEGEKR

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against PSMD12. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PSMD12

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 456 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PSMD12

Target: PSMD12 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 12 (PSMD12))

Alternative Name: PSMD12

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3.
Alias Symbols: MGC75406, p55, Rpn5
Protein Interaction Partner: FUS, HUWE1, SUMO2, UBC, PSMD14, RNF2, KIAA0368, HNRNPA0, TXNL1, SQSTM1, PSMD11, PSMD3, PSMD1, PSMC6, PSMC5, PSMC4, PSMC2, PSMC1, FLNC, FLNB, KCMF1, PARK2, BAG3, RNF11, NPM1, NOS2, PSMD6, PSMD13, PSMD7, UGGT1, PSME3, USP14, PSME1, PSMD10, PSMD8, PSMD4, PSM
Protein Size: 456

Molecular Weight: 53 kDa

Gene ID: 5718

NCBI Accession: NM_002816, NP_002807

UniProt: Q5RBI3

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway