MED7 antibody (Middle Region)

Catalog No. ABIN2787458

This anti-MED7 antibody is a Rabbit Polyclonal antibody detecting MED7 in WB and ChIP. Suitable for Human, Mouse, Dog, Rabbit, Rat, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for MED7 antibody (Middle Region) (ABIN2787458)

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Reactivity: Human, Mouse, Dog, Rabbit, Rat, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED7 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-MED7 Antibody

Binding Specificity: Middle Region

Sequence: KRQRLETAER FQKHLERVIE MIQNCLASLP DDLPHSEAGM RVKTEPMDAD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MED7. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MED7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 233 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED7

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Alternative Name: MED7

Background: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.
Alias Symbols: CRSP33, CRSP9, MGC12284, ARC34
Protein Interaction Partner: HAUS1, CDK8, CDK19, MED13, MED19, MED26, EPAS1, MED6, UBE2I, UL48, SUMO2, UBC, CTDP1, MED10, MED25, MED15, MED14, VDR, SREBF1, RELA, PARP1, ZSCAN1, TRIM15, RHOXF2, PCBD2, MED31, TRIM29, RBFOX2, IKBKG, LZTR1, PML, MED9, MED8, HGS, MED1, HNF4A, ESR2, ESR1,
Protein Size: 233

Molecular Weight: 27 kDa

Gene ID: 9443

NCBI Accession: NM_004270, NP_004261

UniProt: O43513

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha