UBE2K antibody (Middle Region)

Catalog No. ABIN2787511

This anti-UBE2K antibody is a Rabbit Polyclonal antibody detecting UBE2K in WB. Suitable for Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Goat, Horse, Rabbit and Guinea Pig.

Quick Overview for UBE2K antibody (Middle Region) (ABIN2787511)

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Goat, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2K antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2K Antibody

Binding Specificity: Middle Region

Sequence: TVLLSLQALL AAAEPDDPQD AVVANQYKQN PEMFKQTARL WAHVYAGAPV

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE2K. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2K

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 200 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2K

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Alternative Name: UBE2K

Background: UBE2K belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene.
Alias Symbols: DKFZp564C1216, DKFZp686J24237, E2-25K, HIP2, HYPG, LIG, UBC1
Protein Interaction Partner: DDI1, DTX3, MIPOL1, TRIM39, ANAPC11, RNF138, SIAH1, RNF5, REL, FZR1, UBC, UBA1, MARCH7, AMFR, TP53, MDM2, BAG3, PRRG4, IQCB1, HTT, CDC6, WDR1, TYMS, TXN, TXNDC17, WDR61, ITCH, NEDD4L, WWP2, NXF1, NUB1, PIAS4, CASP12, SUMO1, SUMO2, DIABLO, UBE2I, CCNB1, BR
Protein Size: 200

Molecular Weight: 22 kDa

Gene ID: 3093

NCBI Accession: NM_005339, NP_005330

UniProt: P61086