TRIM25 antibody (Middle Region)

Catalog No. ABIN2787550

This anti-TRIM25 antibody is a Rabbit Polyclonal antibody detecting TRIM25 in WB. Suitable for Human, Mouse, Rat, Cow, Guinea Pig, Rabbit and Horse.

Quick Overview for TRIM25 antibody (Middle Region) (ABIN2787550)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRIM25 Antibody

Binding Specificity: Middle Region

Sequence: LLDASETTST RKIKEEEKRV NSKFDTIYQI LLKKKSEIQT LKEEIEQSLT

Predicted Reactivity: Cow: 79%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against TRIM25. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRIM25

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 630 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: TRIM25

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Alias Symbols: EFP, RNF147, Z147, ZNF147
Protein Interaction Partner: UBC, DDX58, SUMO2, AMFR, UBE2D1, SUMO1, PCNA, USP15, UBA1, RPS27A, RPA1, KIF5B, HSPA1L, HSPA1B, HSPA1A, EEF1A2, ACLY, SUZ12, RNF2, EZH2, BMI1, HECW2, TRIM25, TRIM8, TFG, GRIK2, ESR1, OTUB2, USP39, VCP, WARS, UAP1, UBXN6, UBE2S, UBE2D2, TRAF6, ISG15, UBE2D
Protein Size: 630

Molecular Weight: 71 kDa

Gene ID: 7706

NCBI Accession: NM_005082, NP_005073

UniProt: Q14258