CENPH antibody (Middle Region)

Catalog No. ABIN2787617

The Rabbit Polyclonal anti-CENPH antibody has been validated for WB. It is suitable to detect CENPH in samples from Human, Mouse, Rat, Pig, Dog, Horse, Cow, Guinea Pig and Rabbit. There is 1 publication available.

Quick Overview for CENPH antibody (Middle Region) (ABIN2787617)

Target: CENPH (Centromere Protein H (CENPH))

Reactivity: Human, Mouse, Rat, Pig, Dog, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CENPH Antibody

Binding Specificity: Middle Region

Sequence: ESWDLEEKLL DIRKKRLQLK QASESKLLEI QTEKNKQKID LDSMENSERI

Predicted Reactivity: Cow: 93%, Dog: 79%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against CENPH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CENPH

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 247 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CENPH antibody (ABIN2787617)

Liao, Zhang, Zhou, Zimonjic, Mani, Kaba, Gifford, Reinhardt, Popescu, Guo, Eaton, Lodish, Weinberg: "Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity." in: Cancer research, Vol. 67, Issue 17, pp. 8131-8, (2007) (PubMed).

Target Details for CENPH

Target: CENPH (Centromere Protein H (CENPH))

Alternative Name: CENPH

Background: Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. CENPH colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: NNF1, PMF1
Protein Interaction Partner: CENPH, CENPK, MTAP, NDC80, NUF2, SUGT1, UBC, VCP, CENPU, CENPN, CENPA, TRIM36, CENPP, CENPO, CENPI, ELAVL1, KIF2C, PMVK,
Protein Size: 247

Molecular Weight: 27 kDa

Gene ID: 64946

NCBI Accession: NM_022909, NP_075060

UniProt: Q9H3R5