APRT antibody (N-Term)

Catalog No. ABIN2787672

This anti-APRT antibody is a Rabbit Polyclonal antibody detecting APRT in WB and IHC. Suitable for Human, Rat, Dog, Cow and Guinea Pig.

Quick Overview for APRT antibody (N-Term) (ABIN2787672)

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Reactivity: Human, Rat, Dog, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APRT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-APRT Antibody

Binding Specificity: N-Term

Sequence: ADSELQLVEQ RIRSFPDFPT PGVVFRDISP VLKDPASFRA AIGLLARHLK

Predicted Reactivity: Cow: 79%, Dog: 79%, Guinea Pig: 86%, Human: 100%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against APRT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human APRT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 180 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for APRT

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Alternative Name: APRT

Background: Adenine phosphoribosyltransferase (APRT) belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: AMP, DKFZp686D13177, MGC125856, MGC125857, MGC129961
Protein Interaction Partner: UBC, FN1, VCAM1, MLH1, ITGA4, NAPRT, HECW2, GMPS, UMPS, HPRT1, APP, IKBKG, APRT, VHL,
Protein Size: 180

Molecular Weight: 20 kDa

Gene ID: 353

NCBI Accession: NM_000485, NP_000476

UniProt: P07741

Pathways: Ribonucleoside Biosynthetic Process