TUFM antibody (Middle Region)

Catalog No. ABIN2787734

This Rabbit Polyclonal antibody specifically detects TUFM in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiaeand has been mentioned in 1 publication.

Quick Overview for TUFM antibody (Middle Region) (ABIN2787734)

Target: TUFM (Tufm) (Tu Translation Elongation Factor, Mitochondrial (Tufm))

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUFM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TUFM Antibody

Binding Specificity: Middle Region

Sequence: PEKELAMPGE DLKFNLILRQ PMILEKGQRF TLRDGNRTIG TGLVTNTLAM

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 93%, Yeast: 77%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against TUFM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TUFM

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 455 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TUFM antibody (ABIN2787734)

Clemente, Peralta, Cruz-Bermudez, Echevarría, Fontanesi, Barrientos, Fernandez-Moreno, Garesse: "hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria." in: The Journal of biological chemistry, Vol. 288, Issue 12, pp. 8321-31, (2013) (PubMed).

Target Details for TUFM

Target: TUFM (Tufm) (Tu Translation Elongation Factor, Mitochondrial (Tufm))

Alternative Name: TUFM

Background: TUFM is a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: COXPD4, EF-TuMT, EFTU, P43
Protein Interaction Partner: FAM9B, FUNDC1, CMTM5, ARL6IP1, HUWE1, FUS, SUMO2, SUMO3, SPRTN, UBC, MDM2, ASB12, ASB14, ASB10, ASB17, SUZ12, BMI1, RNF2, TUBB2A, XPO1, TUBB6, CAMK1D, NSFL1C, IPO9, UBXN1, DCPS, PROSC, PDIA4, PLAA, GTF3C4, NAE1, PPP5C, PPP1R2, PAFAH1B2, HK1, HEXB, GAPDH,
Protein Size: 455

Molecular Weight: 50 kDa

Gene ID: 7284

NCBI Accession: NM_003321, NP_003312

UniProt: P49411