TUFM antibody

Catalog No. ABIN2855160

The Rabbit Polyclonal anti-TUFM antibody (ABIN2855160) specifically detects TUFM in WB, IF, IP, IHC (p) and ICC. The antibody is reactive with Human samples.

Quick Overview for TUFM antibody (ABIN2855160)

Target: TUFM (Tufm) (Tu Translation Elongation Factor, Mitochondrial (Tufm))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUFM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-TUFM Antibody

Specificity: Knockdown/Knockout validation was supported by references (PMID:26990993)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to TUFM (Tu translation elongation factor, mitochondrial)
TUFM antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human TUFM. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TUFM

Target: TUFM (Tufm) (Tu Translation Elongation Factor, Mitochondrial (Tufm))

Alternative Name: Tu translation elongation factor, mitochondrial

Background: This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17.

Cellular Localization: Mitochondrion

Molecular Weight: 50 kDa

Gene ID: 7284

UniProt: P49411