GCH1 antibody (C-Term)

Catalog No. ABIN2788138

The Rabbit Polyclonal anti-GCH1 antibody has been validated for WB. It is suitable to detect GCH1 in samples from Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Saccharomyces cerevisiae and Zebrafish (Danio rerio).

Quick Overview for GCH1 antibody (C-Term) (ABIN2788138)

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GCH1 Antibody

Binding Specificity: C-Term

Sequence: LRPAGVGVVV EATHMCMVMR GVQKMNSKTV TSTMLGVFRE DPKTREEFLT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 85%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against GCH1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GCH1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 250 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GCH1

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Alternative Name: GCH1

Background: This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described, however, not all variants give rise to a functional enzyme.
Alias Symbols: DYT14, DYT5, DYT5a, GCH, GTP-CH-1, GTPCH1, HPABH4B
Protein Interaction Partner: GCH1, CALML6, CAMKK1, CSNK1G1, CALML5, PACSIN3, RGS19, MAP2K4, MAPK12, MAP2K5, MAPK10, PRKAR2B, PRKAR2A, PPM1B, CSNK2B, CSNK2A1, GIT2, YWHAZ, CNRIP1, RAB26, AHSA1, SP3, RLF, HSPA8, GCHFR, ATP1B1, CDC37, SH3GL2, OSBPL9, RPH3A,
Protein Size: 250

Molecular Weight: 28 kDa

Gene ID: 2643

NCBI Accession: NM_000161, NP_000152

UniProt: P30793