Topoisomerase I antibody (N-Term)

Catalog No. ABIN2788658

Product Details anti-Topoisomerase I Antibody

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SNSEHKDSEK KHKEKEKTKH KDGSSEKHKD KHKDRDKEKR KEEKVRASGD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TOP1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 765 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: TOP1 (TOP1 Products)

Target Type: Viral Protein

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Alias Symbols: TOPI
Protein Interaction Partner: SUMO2, SUMO3, MDM2, UBC, SUMO1, ERG, RNF2, EED, SHFM1, CDK14, TARDBP, CDK2, HDAC9, EIF2AK2, PARP1, WHSC1, VCAM1, ITGA4, IL7R, VCP, PA2G4, NKX3-1, ESR1, RBM39, APP, UTP20, NCSTN, SUPT16H, RBM14, SF3B6, SRSF11, TPR, SNRPD1, S100A9, RPS14, HNRNPM, IK, HNRNPC
Protein Size: 765

Molecular Weight: 91 kDa

Gene ID: 7150

NCBI Accession: NM_003286, NP_003277

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance