Vinculin antibody (C-Term)

Catalog No. ABIN2788672

The Rabbit Polyclonal anti-Vinculin antibody has been validated for WB and IHC. It is suitable to detect Vinculin in samples from Human, Mouse, Rat, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Dog and Guinea Pig.

Quick Overview for Vinculin antibody (C-Term) (ABIN2788672)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Vinculin Antibody

Binding Specificity: C-Term

Sequence: PRPPPPEEKD EEFPEQKAGE VINQPMMMAA RQLHDEARKW SSKGNDIIAA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against VCL. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human VCL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1066 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: VCL

Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Alias Symbols: CMD1W, CMH15, MVCL
Protein Interaction Partner: VCL, UBC, TEKT1, SUMO2, ASB7, CTPS1, GINS3, UBFD1, ISOC1, DCPS, ERP44, PDCD10, FERMT2, YWHAQ, TUBB3, PDIA4, STK24, YWHAZ, YWHAH, YWHAG, YWHAE, XPO1, SNRPF, RAP1GDS1, PPP5C, PPP1R2, PFAS, PDHA1, MSN, HSPA9, HSPA5, GSS, SFN, GNAI3, G6PD, EIF5, ECHS1, TBCB,
Protein Size: 1066

Molecular Weight: 117 kDa

Gene ID: 7414

NCBI Accession: NM_003373, NP_003364

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2