ZAP70 antibody (N-Term)

Catalog No. ABIN2788712

The Rabbit Polyclonal anti-ZAP70 antibody is suitable to detect ZAP70 in samples from Human, Mouse, Rat, Cow and Guinea Pig. It has been validated for WB.

Quick Overview for ZAP70 antibody (N-Term) (ABIN2788712)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ZAP70 Antibody

Binding Specificity: N-Term

Sequence: MRKKQIDVAI KVLKQGTEKA DTEEMMREAQ IMHQLDNPYI VRLIGVCQAE

Predicted Reactivity: Cow: 79%, Guinea Pig: 79%, Human: 100%, Mouse: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against ZAP70. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ZAP70

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 312 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Alias Symbols: FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70
Protein Interaction Partner: UBC, CSK, PLCG1, ZAP70, CD247, CD3E, LAT, NDUFB8, ERBB3, EGFR, ANAPC11, ASB3, PTPN22, NR0B2, SHC1, GRB2, APP, LCK, CBL, CBLC, LCP2, CBLB, PIK3R1, CD38, HSPA4, TUBB, TUBA1A, VAV1, TUBA1B, tat, NFAM1, DEF6, ACP1, LAX1, FCRL3, PTPN3, DBNL, CARD11, SLA2, SLAM
Protein Size: 312

Molecular Weight: 34 kDa

Gene ID: 7535

NCBI Accession: NM_207519, NP_997402

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway