DCTN3 antibody (C-Term)

Catalog No. ABIN2789148

The Rabbit Polyclonal anti-DCTN3 antibody has been validated for WB. It is suitable to detect DCTN3 in samples from Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for DCTN3 antibody (C-Term) (ABIN2789148)

Target: DCTN3 (Dctn3) (Dynactin 3 (p22) (Dctn3))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCTN3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DCTN3 Antibody

Binding Specificity: C-Term

Sequence: ARLQRLAQIH IQQQDQCVEI TEESKALLEE YNKTTMLLSK QFVQWDELLC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against DCTN3. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 186 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DCTN3

Target: DCTN3 (Dctn3) (Dynactin 3 (p22) (Dctn3))

Alternative Name: DCTN3

Background: This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants.
Alias Symbols: DCTN-22, DCTN22, MGC111190
Protein Interaction Partner: LMNB1, FLNB, FHL2, LEO1, TNKS1BP1, NUP85, CCAR2, ACTR10, DCTN2, ACTR1A, UBC, ACTR1B, DYNLT3, ATXN1, GSK3B, Dctn3, Dctn1, Capza1, PBX2,
Protein Size: 186

Molecular Weight: 20 kDa

Gene ID: 11258

NCBI Accession: NM_001248, NP_001239

UniProt: O75355

Pathways: M Phase