MED22 antibody (Middle Region)

Catalog No. ABIN2790421

The Rabbit Polyclonal anti-MED22 antibody has been validated for WB. It is suitable to detect MED22 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for MED22 antibody (Middle Region) (ABIN2790421)

Target: MED22 (Mediator Complex Subunit 22 (MED22))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED22 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED22 Antibody

Binding Specificity: Middle Region

Sequence: SVNEAIDQRN QQLRTLQEEC DRKLITLRDE ISIDLYELEE EYYSSSSSLC

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against MED22. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human MED22

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 200 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED22

Target: MED22 (Mediator Complex Subunit 22 (MED22))

Alternative Name: MED22

Background: This gene is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene.
Alias Symbols: MED24, MGC48682, SURF5
Protein Interaction Partner: CDK19, CDK8, SOX2, UBC, FBXW7, EPAS1, MED4, MED24, MED21, MED1, MED28, MED18, MED12, TADA2A, MED11, MED9, MED30, GOLM1, MED8, MED27, MED17, MED10, THOC7, MED7, MED25, MED29, MED26, MED19,
Protein Size: 200

Molecular Weight: 22 kDa

Gene ID: 6837

NCBI Accession: NM_133640, NP_598395

UniProt: Q15528

Pathways: Regulation of Lipid Metabolism by PPARalpha