RGS10 antibody (Middle Region)

Catalog No. ABIN2792213

The Rabbit Polyclonal anti-RGS10 antibody has been validated for WB and FACS. It is suitable to detect RGS10 in samples from Human, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for RGS10 antibody (Middle Region) (ABIN2792213)

Target: RGS10 (Regulator of G-Protein Signaling 10 (RGS10))

Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RGS10 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-RGS10 Antibody

Binding Specificity: Middle Region

Sequence: DQIFNLMKYD SYSRFLKSDL FLKHKRTEEE EEDLPDAQTA AKRASRIYNT

Predicted Reactivity: Cow: 81%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Rabbit: 90%, Rat: 90%

Characteristics: This is a rabbit polyclonal antibody against RGS10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RGS10

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 181 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RGS10

Target: RGS10 (Regulator of G-Protein Signaling 10 (RGS10))

Alternative Name: RGS10

Background: RGS10 inhibits signal transduction by increasing the GTPASE activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It associates specifically with the activated forms of the G protein subunits G(I)-ALPHA and G(Z)- alpha but fails to interact with the structurally and functionally distinct G(S)-alpha subunit. Activity on G(Z)-alpha is inhibited by palmitoylation of the G-protein.
Protein Interaction Partner: UBC, ADRB2, APP, PRKACA, GNAI3, GNAO1, GNAI1, GNAZ, GNRHR, CALM1, ACP6, SAP18, EIF6,
Protein Size: 181

Molecular Weight: 21 kDa

Gene ID: 6001

NCBI Accession: NM_001005339, NP_001005339

UniProt: Q96GN0

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling