NAPA antibody (N-Term)

Catalog No. ABIN2792279

This anti-NAPA antibody is a Rabbit Polyclonal antibody detecting NAPA in WB. Suitable for Mouse, Cow, Dog and Zebrafish (Danio rerio).

Quick Overview for NAPA antibody (N-Term) (ABIN2792279)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivity: Mouse, Cow, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAPA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NAPA Antibody

Binding Specificity: N-Term

Sequence: MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN

Predicted Reactivity: Cow: 100%, Dog: 100%, Mouse: 78%, Zebrafish: 78%

Characteristics: This is a rabbit polyclonal antibody against NAPA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NAPA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 295 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Alternative Name: NAPA

Background: The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37 % , 60 %, and 67 % identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively.
Alias Symbols: SNAPA
Protein Interaction Partner: UBC, RPA3, RPA2, RPA1, RNF2, ATP4A, STX5, ITGA4, FN1, VCAM1, VCP, DHX40, DHX29, THUMPD3, STAM2, VAMP2, APP, NSF, TRPC3, RTN4, STX12, GNA12, STX8, GOSR2, GOSR1, BNIP1, STX7, VAMP8, SNAP23, STX4, STX1A, SNAP25,
Protein Size: 295

Molecular Weight: 33 kDa

Gene ID: 8775

NCBI Accession: NM_003827, NP_003818

UniProt: P54920

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization