GLI1 antibody (N-Term)

Catalog No. ABIN2792532

The Rabbit Polyclonal anti-GLI1 antibody has been validated for WB. It is suitable to detect GLI1 in samples from Human, Dog, Guinea Pig, Horse, Rabbit and Cow.

Quick Overview for GLI1 antibody (N-Term) (ABIN2792532)

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Reactivity: Human, Dog, Guinea Pig, Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLI1 Antibody

Binding Specificity: N-Term

Sequence: LRPLPSQGAP SVGTEGLSGP PFCHQANLMS GPHSYGPARE TNSCTEGPLF

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human

Predicted Reactivity: Cow: 92%, Dog: 92%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Rabbit: 100%

Characteristics: This is a rabbit polyclonal antibody against GLI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLI1

Application Details

Application Notes: WB Suggested Anti-GLI1 Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1106 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: GLI1

Background: GLI1 is a protein which is a member of the Kruppel family of zinc finger proteins. The function of this gene has not been determined, however, it may play a role in normal development gene transcription. Mouse mutation studies indicate possible involvement in human foregut malformation.
Alias Symbols: GLI
Protein Interaction Partner: TRIM42, GCC1, PRKAA2, VHL, FEM1B, CDK4, UBC, MYC, ITCH, NUMB, SUFU, RAI1, XBP1, HDAC2, HDAC1, STK36, DYRK1A, ZIC2, ZIC1, Sin3a, Shh, SAP18, Su(fu), Pias1,
Protein Size: 1106

Molecular Weight: 118 kDa

Gene ID: 2735

NCBI Accession: NM_005269, NP_005260

UniProt: P08151

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis