GLI1 antibody (N-Term)
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- Target See all GLI1 Antibodies
- GLI1 (Zinc Finger Protein GLI1 (GLI1))
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Binding Specificity
- N-Term
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Reactivity
- Human, Dog, Guinea Pig, Horse, Rabbit, Cow
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GLI1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- LRPLPSQGAP SVGTEGLSGP PFCHQANLMS GPHSYGPARE TNSCTEGPLF
- Cross-Reactivity
- Cow (Bovine), Dog (Canine), Human
- Predicted Reactivity
- Cow: 92%, Dog: 92%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Rabbit: 100%
- Characteristics
- This is a rabbit polyclonal antibody against GLI1. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Protein A purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human GLI1
- Top Product
- Discover our top product GLI1 Primary Antibody
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- Application Notes
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WB Suggested Anti-GLI1 Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator. - Comment
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Antigen size: 1106 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- GLI1 (Zinc Finger Protein GLI1 (GLI1))
- Alternative Name
- GLI1 (GLI1 Products)
- Background
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GLI1 is a protein which is a member of the Kruppel family of zinc finger proteins. The function of this gene has not been determined, however, it may play a role in normal development gene transcription. Mouse mutation studies indicate possible involvement in human foregut malformation.
Alias Symbols: GLI
Protein Interaction Partner: TRIM42, GCC1, PRKAA2, VHL, FEM1B, CDK4, UBC, MYC, ITCH, NUMB, SUFU, RAI1, XBP1, HDAC2, HDAC1, STK36, DYRK1A, ZIC2, ZIC1, Sin3a, Shh, SAP18, Su(fu), Pias1,
Protein Size: 1106 - Molecular Weight
- 118 kDa
- Gene ID
- 2735
- NCBI Accession
- NM_005269, NP_005260
- UniProt
- P08151
- Pathways
- Hedgehog Signaling, Dopaminergic Neurogenesis
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