MAF antibody (Middle Region)

Catalog No. ABIN2792536

The Rabbit Polyclonal anti-MAF antibody (ABIN2792536) specifically detects MAF in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Horse, Guinea Pig and Pig samples.

Quick Overview for MAF antibody (Middle Region) (ABIN2792536)

Target: MAF (V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian) (MAF))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Horse, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAF Antibody

Binding Specificity: Middle Region

Sequence: LQQVDHLKQE ISRLVRERDA YKEKYEKLVS SGFRENGSSS DNPSSPEFFI

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 78%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MAF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MAF

Application Details

Application Notes: WB Suggested Anti-MAF Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: Hela cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 403 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MAF

Target: MAF (V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian) (MAF))

Alternative Name: MAF

Background: MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. It recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. MAF is also involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It may be involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Alias Symbols: MGC71685, c-MAF
Protein Interaction Partner: MAFB, FOSL1, MAF, FOS, ATF4, AHR, SUMO1, UBE2I, PML, SIN3A, HDAC2, SMARCA5, CEBPA, KDM5B, MAFA, SOX9, EP300, MAFG, USF2, CREBBP, MYB, JUN, ETS1, HOXD12,
Protein Size: 403

Molecular Weight: 42 kDa

Gene ID: 4094

NCBI Accession: NM_005360, NP_005351