NFX1 antibody (Middle Region)

Catalog No. ABIN2792551

The Rabbit Polyclonal anti-NFX1 antibody is suitable to detect NFX1 in samples from Human, Mouse, Rat, Dog, Rabbit and Cow. It has been validated for WB and IHC.

Quick Overview for NFX1 antibody (Middle Region) (ABIN2792551)

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NFX1 Antibody

Binding Specificity: Middle Region

Sequence: TLTGVLEREM QARPPPPIPH HRHQSDKNPG SSNLQKITKE PIIDYFDVQD

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 85%, Dog: 85%, Human: 100%, Mouse: 76%, Rabbit: 85%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against NFX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFX1

Application Details

Application Notes: WB Suggested Anti-NFX1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1120 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFX1

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Alternative Name: NFX1

Background: MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.
Alias Symbols: DKFZp779G2416, MGC20369, NFX2
Protein Interaction Partner: UBC, BMI1, HECW2, SRSF4, SRSF3, RPS19, RPS3A, RPL22, RPL17, RPL13, RPL7A, RPL6, RPL3, PABPC3, YBX1, NCL, HNRNPM, HSPA1A, HNRNPU, HNRNPH1, HNRNPA2B1, HIST1H2AE, EEF1A2, DMD, DDX5, FAM208B, PABPC1, RPL13A, LARP1, APC2, MATR3, HIST1H4E, USP9X, SIN3A, MAML3,
Protein Size: 1120

Molecular Weight: 124 kDa

Gene ID: 4799

NCBI Accession: NM_002504, NP_002495

UniProt: Q12986