NFYC antibody (C-Term)

Catalog No. ABIN2792552

This Rabbit Polyclonal antibody specifically detects NFYC in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse and Pig.

Quick Overview for NFYC antibody (C-Term) (ABIN2792552)

Target: NFYC (Nuclear Transcription Factor Y, gamma (NFYC))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NFYC Antibody

Binding Specificity: C-Term

Sequence: MTSLSPLHPS QQLYQIQQVT MPAGQDLAQP MFIQSANQPS DGQAPQVTGD

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 92%, Pig: 92%, Rabbit: 85%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against NFYC. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NFYC

Application Details

Application Notes: WB Suggested Anti-NFYC Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 458 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFYC

Target: NFYC (Nuclear Transcription Factor Y, gamma (NFYC))

Alternative Name: NFYC

Background: NFYC is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. NFYC forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity.
Alias Symbols: HSM, CBFC, HAP5, CBF-C, NF-YC, H1TF2A
Protein Interaction Partner: NFYA, CDK2, UBC, SNX2, NFYB, Cebpb, ELAVL1, GAPDH, KAT2B, TP73, TAF12, TAF11, TAF6, TBPL2, TRIM27, HDAC1, KIAA2018, ARID2, GLTSCR1, GLTSCR1L, ZDHHC17, CIC, POGZ, ATF6, ADNP2, LMO4, SREBF2, SP1, DR1, CSN2, DST, SMAD3, SMAD2, ATF6B, MYC, TBP,
Protein Size: 458

Molecular Weight: 50 kDa

Gene ID: 4802

NCBI Accession: NM_001142587

UniProt: Q13952

Pathways: Regulation of Lipid Metabolism by PPARalpha