RFX1 antibody (C-Term)

Catalog No. ABIN2792556

This Rabbit Polyclonal antibody specifically detects RFX1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig and Zebrafish (Danio rerio).

Quick Overview for RFX1 antibody (C-Term) (ABIN2792556)

Target: RFX1 (Regulatory Factor X, 1 (Influences HLA Class II Expression) (RFX1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RFX1 Antibody

Binding Specificity: C-Term

Sequence: ESEDELPQDI SLAAGGESPA LGPETLEPPA KLARTDARGL FVQALPSS

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 92%, Pig: 92%, Rat: 92%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against RFX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RFX1

Application Details

Application Notes: WB Suggested Anti-RFX1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: MCF7 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 979 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RFX1

Target: RFX1 (Regulatory Factor X, 1 (Influences HLA Class II Expression) (RFX1))

Alternative Name: RFX1

Background: RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX1 is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes.This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: EF-C, EFC, RFX
Protein Interaction Partner: SOX2, SMAD9, SMAD4, SMAD1, NOTCH1, Cebpb, NCOA3, HDAC1, NFIC, NFIB, TADA3, RFX3, MAGED1, ABL1, NFIX, HMGB1, HIVEP2, RFX2, ADD1,
Protein Size: 979

Molecular Weight: 105 kDa

Gene ID: 5989

NCBI Accession: NM_002918, NP_002909

UniProt: P22670

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response