GTF2E1 antibody (N-Term)

Catalog No. ABIN2792622

The Rabbit Polyclonal anti-GTF2E1 antibody (ABIN2792622) specifically detects GTF2E1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for GTF2E1 antibody (N-Term) (ABIN2792622)

Target: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2E1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GTF2E1 Antibody

Binding Specificity: N-Term

Sequence: DHMRRRIETD ERDSTNRASF KCPVCSSTFT DLEANQLFDP MTGTFRCTFC

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 92%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against GTF2E1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GTF2E1

Application Details

Application Notes: WB Suggested Anti-GTF2E1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 439 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GTF2E1

Target: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Alternative Name: GTF2E1 / TF2E1

Background: GTF2E1 belongs to the TFIIE alpha subunit family. It recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Alias Symbols: FE, TF2E1, TFIIE-A
Protein Interaction Partner: UBC, AARSD1, ACBD3, XPO5, SH3GLB2, OXR1, NSUN2, RPUSD2, SNF8, EFTUD2, TTC1, SH3GL1, SARS, HARS, SRPK2, TCEA1, GTF2H1, GTF2E2, CDK7, APP, tat, GTF2F1, ELAVL1, ZSCAN1, ZBTB9, TRIM15, PCBD2, TRIM29, CBFA2T2, IKBKG, ATF7IP, TAF6, GTF2B, TRIM24, SND1, GTF2H4,
Protein Size: 439

Molecular Weight: 49 kDa

Gene ID: 2960

NCBI Accession: NM_005513, NP_005504

UniProt: P29083