EN1 antibody (C-Term)

Catalog No. ABIN2792656

The Rabbit Polyclonal anti-EN1 antibody has been validated for WB. It is suitable to detect EN1 in samples from Guinea Pig and Rabbit. There is 1 publication available.

Quick Overview for EN1 antibody (C-Term) (ABIN2792656)

Target: EN1 (Engrailed Homeobox 1 (EN1))

Reactivity: Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EN1 Antibody

Binding Specificity: C-Term

Sequence: LMGSANGGPV VKTDSQQPLV WPAWVYCTRY SDRPSSGPRT RKLKKKKNEK

Cross-Reactivity: Chicken, Human, Mouse (Murine)

Predicted Reactivity: Guinea Pig: 93%, Rabbit: 86%

Characteristics: This is a rabbit polyclonal antibody against EN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EN1

Application Details

Application Notes: WB Suggested Anti-EN1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 392 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EN1 antibody (ABIN2792656)

Beltran, Graves, Blancafort: "Novel role of Engrailed 1 as a prosurvival transcription factor in basal-like breast cancer and engineering of interference peptides block its oncogenic function." in: Oncogene, Vol. 33, Issue 39, pp. 4767-77, (2014) (PubMed).

Target Details for EN1

Target: EN1 (Engrailed Homeobox 1 (EN1))

Alternative Name: Engrailed-1 / EN1

Background: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: -
Protein Interaction Partner: TLE1, PAX6, JUN,
Protein Size: 392

Molecular Weight: 40 kDa

Gene ID: 2019

NCBI Accession: NM_001426, NP_001417

UniProt: Q05925

Pathways: Dopaminergic Neurogenesis