Id2 antibody (Middle Region)

Catalog No. ABIN2792664

This Rabbit Polyclonal antibody specifically detects Id2 in IHC and WB. It exhibits reactivity toward Human, Rat, Mouse, Cow and Pig.

Quick Overview for Id2 antibody (Middle Region) (ABIN2792664)

Target: Id2 (Inhibitor of DNA Binding 2 (Id2))

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Id2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-Id2 Antibody

Binding Specificity: Middle Region

Sequence: TIVSLHHQRP GQNQASRTPL TTLNTDISIL SLQASEFPSE LMSNDSKALC

Cross-Reactivity: Cow (Bovine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Human: 100%, Mouse: 100%, Pig: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ID2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ID2

Application Details

Application Notes: WB Suggested Anti-ID2 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: Human Thymus.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 134 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Id2

Target: Id2 (Inhibitor of DNA Binding 2 (Id2))

Alternative Name: ID2 / BHLHB26

Background: ID2 belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation.The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GIG8, ID2A, ID2H, MGC26389, bHLHb26
Protein Interaction Partner: ASB4, UBC, SUV39H2, PRMT6, KDM1A, SUV39H1, TCF12, TCF3, TSTA3, MAPK8, ID2, HSPA5, RBL2, RBL1, RB1, CDK2, USP1, MAPK3, MAPK1, E2F4, SIN3A, GATA4, NKX2-5, LRIF1, PPP1CA, RBM48, NEDD9, UNC119, MSC, TCF4, ELK3, ELK4, ELK1, PAX8, PAX5, PAX2, MYF6, MYF5, MYOG,
Protein Size: 134

Molecular Weight: 15 kDa

Gene ID: 3398

NCBI Accession: NM_002166, NP_002157

UniProt: Q02363