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Nucleobindin 1 antibody (C-Term)

This anti-Nucleobindin 1 antibody is a Rabbit Polyclonal antibody detecting Nucleobindin 1 in IHC. Suitable for Mouse, Rat and Dog. This Primary Antibody has been cited in 1 publication.
Catalog No. ABIN2792697

Quick Overview for Nucleobindin 1 antibody (C-Term) (ABIN2792697)

Target

See all Nucleobindin 1 (NUCB1) Antibodies
Nucleobindin 1 (NUCB1)

Reactivity

  • 36
  • 21
  • 20
  • 1
Mouse, Rat, Dog

Host

  • 37
  • 7
Rabbit

Clonality

  • 39
  • 5
Polyclonal

Conjugate

  • 30
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Nucleobindin 1 antibody is un-conjugated

Application

  • 43
  • 18
  • 14
  • 13
  • 13
  • 11
  • 8
  • 6
  • 5
  • 3
  • 2
Immunohistochemistry (IHC)
  • Binding Specificity

    • 15
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Sequence

    SQPDGQLQFR ADTGDAPVPA PAGDQKDVPA SEKKVPEQPP VLPQLDSQHL

    Cross-Reactivity

    Dog (Canine), Mouse (Murine), Rat (Rattus)

    Predicted Reactivity

    Dog: 78%, Mouse: 92%, Rat: 100%

    Characteristics

    This is a rabbit polyclonal antibody against NUCB1. It was validated on Western Blot and immunohistochemistry.

    Purification

    Protein A purified

    Immunogen

    The immunogen is a synthetic peptide corresponding to a region of Rat
  • Application Notes

    WB Suggested Anti-NUCB1 Antibody Titration: 5.0 µg/mL
    Positive Control: Hela cell lysate.
    Optimal working dilutions should be determined experimentally by the investigator.

    Comment

    Antigen size: 459 AA

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    -20 °C

    Storage Comment

    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Lin, Li, Zhang, Huang, Tong, Farquhar, Xu: "Calnuc binds to Alzheimer's beta-amyloid precursor protein and affects its biogenesis." in: Journal of neurochemistry, Vol. 100, Issue 6, pp. 1505-14, (2007) (PubMed).

  • Target

    Nucleobindin 1 (NUCB1)

    Alternative Name

    Nucleobindin-1

    Background

    An extracellular calcium binding protein of the mineralized matrix of bone [Calnuc or RGD:620030]. Also useful as a Golgi marker in immunohistochemistry (1:100 dilution).
    Alias Symbols: Nucb
    Protein Interaction Partner: STXBP5L, GNAI3,
    Protein Size: 459

    Molecular Weight

    54 kDa

    Gene ID

    84595

    NCBI Accession

    NM_053463, NP_445915

    UniProt

    Q63083
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