Nucleobindin 1 antibody (C-Term)

Catalog No. ABIN2792698

This Rabbit Polyclonal antibody specifically detects Nucleobindin 1 in WB. It exhibits reactivity toward Human and has been mentioned in 6+ publications.

Quick Overview for Nucleobindin 1 antibody (C-Term) (ABIN2792698)

Target: Nucleobindin 1 (NUCB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleobindin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nucleobindin 1 Antibody

Binding Specificity: C-Term

Sequence: PAAHPEGQLK FHPDTDDVPV PAPAGDQKEV DTSEKKLLER LPEVEVPQHL

Cross-Reactivity: Human

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against NUCB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NUCB1

Application Details

Application Notes: WB Suggested Anti-NUCB1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 461 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Nucleobindin 1 antibody (ABIN2792698)

Bonito-Oliva, Barbash, Sakmar, Graham: "Nucleobindin 1 binds to multiple types of pre-fibrillar amyloid and inhibits fibrillization." in: Scientific reports, Vol. 7, pp. 42880, (2017) (PubMed).

Larkin, Ribeiro, Lavoie: "Topology and membrane anchoring of the lysosomal storage disease-related protein CLN5." in: Human mutation, Vol. 34, Issue 12, pp. 1688-97, (2013) (PubMed).

Lin, Fischer, Lavoie, Huang, Farquhar: "Calnuc plays a role in dynamic distribution of Galphai but not Gbeta subunits and modulates ACTH secretion in AtT-20 neuroendocrine secretory cells." in: Molecular neurodegeneration, Vol. 4, pp. 15, (2009) (PubMed).

Valencia, Cotten, Duan, Liu: "Modulation of nucleobindin-1 and nucleobindin-2 by caspases." in: FEBS letters, Vol. 582, Issue 2, pp. 286-90, (2008) (PubMed).

Sugeno, Takeda, Hasegawa, Kobayashi, Kikuchi, Mori, Wakabayashi, Itoyama: "Serine 129 phosphorylation of alpha-synuclein induces unfolded protein response-mediated cell death." in: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23179-88, (2008) (PubMed).

Lin, Li, Zhang, Huang, Tong, Farquhar, Xu: "Calnuc binds to Alzheimer's beta-amyloid precursor protein and affects its biogenesis." in: Journal of neurochemistry, Vol. 100, Issue 6, pp. 1505-14, (2007) (PubMed).

Target Details for Nucleobindin 1

Target: Nucleobindin 1 (NUCB1)

Alternative Name: Nucleobindin-1

Background: Nucleobindin, also known as calnuc, participates in Ca2+ storage in the Golgi, as well as in other biological processes that involve DNA-binding and protein-protein interactions.
Alias Symbols: NUC, CALNUC
Protein Interaction Partner: REL, UBC, MDM2, CTPS2, RAD23B, PRKDC, METTL1, GTF2I, CBS, XPNPEP1, WARS, PTGS2, XPO1, SET, RBL1, MYC, HIVEP1, GNAS, GNAI3, GNAI1, KLC4, PTGS1, NDN, GNAI2, GNAO1, GNAZ,
Protein Size: 461

Molecular Weight: 54 kDa

Gene ID: 4924

NCBI Accession: NM_006184, NP_006175

UniProt: Q02818