SMAD4 antibody (pThr277)

Catalog No. ABIN2794149

This anti-SMAD4 antibody is a Rabbit Polyclonal antibody detecting SMAD4 in IHC, IF and DB. Suitable for Human.

Quick Overview for SMAD4 antibody (pThr277) (ABIN2794149)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Dot Blot (DB)

Product Details anti-SMAD4 Antibody

Binding Specificity: pThr277

Purpose: Rabbit Anti-Human Phospho-SMAD4(T277) Antibody

Immunogen: This SMAD4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4.

Isotype: Ig Fraction

Application Details

Application Notes: Immunofluorescence, Dot Blot, Immunohistochemistry
Recommended Dilutions
IF: 1:10-50, DB: 1:500, IHC: 1:10-50Confocal immunofluorescent analysis of Phospho-SMAD4-T277 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background:

Target Description: Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

Gene Symbol: SMAD4

Molecular Weight: 60439 Da

Gene ID: 4089

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy