Deoxyuridine Triphosphatase (DUT) (AA 170-198), (C-Term) antibody

Catalog No. ABIN2798480

The Rabbit Polyclonal anti- antibody has been validated for WB and IHC. It is suitable to detect in samples from Human.

Quick Overview for Deoxyuridine Triphosphatase (DUT) (AA 170-198), (C-Term) antibody (ABIN2798480)

Target: Deoxyuridine Triphosphatase (DUT)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 170-198, C-Term

Purpose: Rabbit Anti-Human DUT (C-term) Antibody

Immunogen: This DUT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the C-terminal region of human DUT.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50Western blot analysis of lysate from A431 cell line, using DUT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details

Target: Deoxyuridine Triphosphatase (DUT)

Alternative Name: DUT

Background:

Target Description: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Gene Symbol: DUT

Molecular Weight: 26563 Da

Gene ID: 1854

UniProt: P33316