Nanog antibody (Center)

Catalog No. ABIN2798793

This anti-Nanog antibody is a Rabbit Polyclonal antibody detecting Nanog in WB, IF, IHC and FACS. Suitable for Human.

Quick Overview for Nanog antibody (Center) (ABIN2798793)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Nanog Antibody

Binding Specificity: AA 94-123, Center

Purpose: Rabbit Anti-Human NANOG (Center) Antibody

Immunogen: This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-123 amino acids from the Central region of human NANOG.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50, IF: 1:10-50, FC: 1:10-50NANOG Antibody (Center) . FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background:

Target Description: NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.

Gene Symbol: NANOG

Molecular Weight: 34620 Da

Gene ID: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance