Nanog antibody (AA 267-292)

Catalog No. ABIN2798794

This Rabbit Polyclonal antibody specifically detects Nanog in WB and IF. It exhibits reactivity toward Human.

Quick Overview for Nanog antibody (AA 267-292) (ABIN2798794)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Nanog Antibody

Binding Specificity: AA 267-292

Purpose: Rabbit Anti-Human NANOG (S285) Antibody

Immunogen: This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-292 amino acids from human NANOG.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunofluorescence
Recommended Dilutions
WB: 1:1000, IF: 1:10-50Western blot analysis of NANOG (arrow) using rabbit NANOG Antibody (S285) . Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.310 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background:

Target Description: NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.

Gene Symbol: NANOG

Molecular Weight: 34620 Da

Gene ID: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance