CD33 antibody (N-Term)

Catalog No. ABIN2798973

This anti-CD33 antibody is a Rabbit Polyclonal antibody detecting CD33 in WB and IHC. Suitable for Human.

Quick Overview for CD33 antibody (N-Term) (ABIN2798973)

Target: CD33

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD33 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CD33 Antibody

Binding Specificity: AA 12-39, N-Term

Purpose: Rabbit Anti-Human CD33 (SIGLEC3) (N-term) Antibody

Immunogen: This CD33 (SIGLEC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human CD33 (SIGLEC3).

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50The anti-Siglec3 N-term Pab , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for CD33

Target: CD33

Alternative Name: CD33

Background:

Target Description: SIGLEC3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,6-linked sialic acid, the sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, SIGLEC3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. This protein Induces apoptosis in acute myeloid leukemia (in vitro). It has been shown to interact with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. SIGLEC3 expresses in monocytic/myeloid lineage cells, and contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. The gene for SIGLEC3 belongs to the immunoglobulin superfamily.

Gene Symbol: CD33

Molecular Weight: 39825 Da

Gene ID: 945

UniProt: P20138