HDAC9 antibody (C-Term)

Catalog No. ABIN2800619

The Rabbit Polyclonal anti-HDAC9 antibody has been validated for WB, IHC and IP. It is suitable to detect HDAC9 in samples from Human.

Quick Overview for HDAC9 antibody (C-Term) (ABIN2800619)

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-HDAC9 Antibody

Binding Specificity: AA 503-533, C-Term

Purpose: Rabbit Anti-Human HDAC9 (C-term) Antibody

Immunogen: This HDAC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-533 amino acids from the C-terminal region of human HDAC9.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100Both anti-HDAC9 N-term (102-10933) and C-term (102-10934) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone. Bottom figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated. BC = breast carcinoma, HC = hepatocarcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.000 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for HDAC9

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Alternative Name: HDAC9

Background:

Target Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.

Gene Symbol: HDAC9

Molecular Weight: 111297 Da

Gene ID: 9734

UniProt: Q9UKV0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development