LAMP3 antibody (N-Term)

Catalog No. ABIN2800632

This anti-LAMP3 antibody is a Rabbit Polyclonal antibody detecting LAMP3 in WB and IF. Suitable for Human.

Quick Overview for LAMP3 antibody (N-Term) (ABIN2800632)

Target: LAMP3 (Lysosomal-Associated Membrane Protein 3 (LAMP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAMP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-LAMP3 Antibody

Binding Specificity: AA 8-38, N-Term

Purpose: Rabbit Anti-Human LAMP3 (N-term) Antibody

Immunogen: This LAMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-38 amino acids from the N-terminal region of human LAMP3.

Isotype: Ig Fraction

Application Details

Application Notes: Immunofluorescence, Western Blot
Recommended Dilutions
IF: 1:100, WB: 1:1000Fluorescent image of U251 cells stained with LAMP3 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4 % PFA (20 min), permeabilized with Triton X-100 (0.2 %, 30 min). Cells were then incubated with 102-13764 LAMP3 (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/mL, 5 min). LAMP3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.LAMP3 Antibody (N-term) .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for LAMP3

Target: LAMP3 (Lysosomal-Associated Membrane Protein 3 (LAMP3))

Alternative Name: LAMP3

Background:

Target Description: LAMP3 is a member of a family of membrane glycoproteins. It may change lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells.

Gene Symbol: LAMP3

Molecular Weight: 44346 Da

Gene ID: 27074

UniProt: Q9UQV4