SMYD2A antibody (AA 351-433)

Catalog No. ABIN2803151

This Rabbit Polyclonal antibody specifically detects SMYD2A in WB, ELISA, IF (p), IHC (p), ICC, IF (cc) and IHC (fro). It exhibits reactivity toward Human and Mouse.

Quick Overview for SMYD2A antibody (AA 351-433) (ABIN2803151)

Target: SMYD2A (SET and MYND Domain Containing 2a (SMYD2A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMYD2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SMYD2A Antibody

Binding Specificity: AA 351-433

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SMYD2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SMYD2A

Target: SMYD2A (SET and MYND Domain Containing 2a (SMYD2A))

Alternative Name: SMYD2

Background:

Synonyms: KMT3C, HSKM-B, ZMYND14, N-lysine methyltransferase SMYD2, Histone methyltransferase SMYD2, Lysine N-methyltransferase 3C, SET and MYND domain-containing protein 2, SMYD2

Background: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.

Gene ID: 56950

UniProt: Q9NRG4