SIRT7 antibody (AA 231-330) (AbBy Fluor® 594)

Catalog No. ABIN2806390

The Rabbit Polyclonal anti-SIRT7 antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect SIRT7 in samples from Mouse.

Quick Overview for SIRT7 antibody (AA 231-330) (AbBy Fluor® 594) (ABIN2806390)

Target: SIRT7 (Sirtuin 7 (SIRT7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT7 antibody is conjugated to AbBy Fluor® 594

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SIRT7 Antibody (AbBy Fluor® 594)

Binding Specificity: AA 231-330

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SIRT7

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SIRT7

Target: SIRT7 (Sirtuin 7 (SIRT7))

Alternative Name: SIRT7

Background:

Synonyms: SIR2L7, NAD-dependent protein deacetylase sirtuin-7, Regulatory protein SIR2 homolog 7, SIR2-like protein 7, SIRT7

Background: NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.

Gene ID: 51547

UniProt: Q9NRC8