TRIM32 antibody (AA 301-400) (AbBy Fluor® 594)

Catalog No. ABIN2807199

This Rabbit Polyclonal antibody specifically detects TRIM32 in WB, FACS, IF (cc) and IF (p). It exhibits reactivity toward Mouse.

Quick Overview for TRIM32 antibody (AA 301-400) (AbBy Fluor® 594) (ABIN2807199)

Target: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM32 antibody is conjugated to AbBy Fluor® 594

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-TRIM32 Antibody (AbBy Fluor® 594)

Binding Specificity: AA 301-400

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse TRIM32

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TRIM32

Target: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Alternative Name: TRIM32/BBS11

Background:

Synonyms: 72 kda Tat interacting Protein, BBS11, HT2A, LGMD2H, Limb girdle muscular dystrophy 2H autosomal recessive, Limb girdle muscular dystrophy 2H, Muscular dystrophy Hutterite type, TAT interactive protein 72KD, TATIP, Tripartite Mot Containing Protein 32, Zinc Finger Protein HT2A, TRI32_MOUSE.

Background: Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) protein family. TRIM32, like all TRIM proteins, contains a domain structure composed of a B-box, a RING-finger and a coiled-coil motif. Additionally, TRIM32 has six C-terminal NHL domains, it is expressed mainly in the skeletal muscle. The TRIM32 gene encodes an E3 ubiquitin ligase, a protein that attaches ubiquitin to a lysine residue on a target protein and acts in conjunction with ubiquitin-conjugating enzymes UbcH5a, UbcH5c and UbcH6. Mutations in the TRIM32 gene cause two forms of autosomal recessive muscular dystrophy designated limb girdle muscular dystrophy type 2H (LGMD2H) and sarcotubular myopathy (STM). TRIM32 mutations can also result in Bardet-Biedl syndrome (BBS), an autosomal recessive disorder characterized by pigmentary retinopathy, polydactyly, hypogenitalism, renal abnormalities, learning disabilities and obesity.

Gene ID: 22954

Pathways: Negative Regulation of intrinsic apoptotic Signaling